Hybribio HybriMax? makes use of both DNA amplification and Hybribio’s proprietary Flow-through Hybridization Technology to identify HPV, STDs, Thalassemia target DNA using specific probes with gene-chip technology.

Macro-array Flow-through Hybridization Format

Flow-through Hybridization technology is a perfect platform for a cost-effective low-density Macro-array, a potential replacement for the current expensive and complicated DNA Micro-array.

Advantages

–           Reduced hybridization duration from hours down to minutes
–           Require much less sample and reagent volumes
–           Cost effective
–           Simple signal detection methodology

US Patent: 5,741,647 / 6,020,187

Flow-through Hybridization Technology

The revolution brought about by molecular biology depended heavily on nucleic acid hybridization procedures. Hybridizations are used extensively in laboratories focusing on detecting specific nucleotide sequences in DNA based hybridization assays and the detections of genetic mutation are increasingly being applied in life-sciences.

The “Flow-through Hybridization” concept is simple and easy, it provides optimal conditions for effective hybridizations.

The principle of the “Flow-through Hybridization” invention is based on directing the flow of the reacting molecules towards the target probes (immobilized on the membrane) in a pro-active manner, when the probes and targets are complementary to each other and they will subsequently form duplexes.

When comparing with conventional method, this “Flow-through Hybridization” technology increases the molecular binding rate by thousand folds, thus allowing an efficient molecular annealing or hybridization to occur. Flow-Through Hybridization platform also has an improvement on enhancing molecular interaction occurs in three-dimensional volumes rather than the conventional two-dimensional surface.